Coding

Part:BBa_K3286105:Design

Designed by: Robert Hooftman   Group: iGEM19_Wageningen_UR   (2019-10-15)


Hypothetical protein PD1764 of X. fastidiosa


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 850
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Some modifications were made in gc content to make ordering easier. Start codon is removed so it can be used in BBa_K3286100 for production. When using it without tags, add start codon.

Source

This part was taken from the genome, but ordered as gBlock, for which some modifications were made in GC content.

References

[1] F. Labroussaa, A. R. Zeilinger, and R. P. P. Almeida, “Blocking the Transmission of a Noncirculative Vector-Borne Plant Pathogenic Bacterium,” Mol. Plant-Microbe Interact., vol. 29, no. 7, pp. 535–544, Jul. 2016.