Coding
Part:BBa_K3286105:Design
Designed by: Robert Hooftman Group: iGEM19_Wageningen_UR (2019-10-15)
Hypothetical protein PD1764 of X. fastidiosa
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 850
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 357
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Some modifications were made in gc content to make ordering easier. Start codon is removed so it can be used in BBa_K3286100 for production. When using it without tags, add start codon.
Source
This part was taken from the genome, but ordered as gBlock, for which some modifications were made in GC content.
References
[1] F. Labroussaa, A. R. Zeilinger, and R. P. P. Almeida, “Blocking the Transmission of a Noncirculative Vector-Borne Plant Pathogenic Bacterium,” Mol. Plant-Microbe Interact., vol. 29, no. 7, pp. 535–544, Jul. 2016.